Banner background

Genome assembly

Species

Parnassius mnemosyne

Clouded apollo butterfly

Description

  • Assembly Name: Parnassius_mnemosyne_n_2023_11
  • Assembly Type: Haploid
  • Assembly Level: Scaffold
  • Genome Representation: Full
  • Accession: GCA_963668995.1

Assembly Statistics

StatisticValue
Assembly length (Mbp)1494.39
GC %38.04
Contig #285
Contig N50 (Mbp)23.93
Contig L5022
Contig N90 (Mbp)4.73
Contig L9082
Scaffold #169
Scaffold N50 (Mbp)49.64
Scaffold L5013
Scaffold N90 (Mbp)34.77
Scaffold L9027
Scaffolds >= 10 kb168
BUSCO % (lepidoptera_odb10)C:99 [S:97.8, D:1.2], F:0.2, M:0.8, n:5286

Annotation Statistics

StatisticValue
Gene #22852
Transcript #27376
Average exons per transcript4.7
Avg. gene length (bp)16898
Avg. transcript length (bp)16026
Avg. exon length (bp)343
Avg. intron length (bp)3916

Notes: Assembly statistics were calculated for the primary genome assembly GCA_963668995.1 (CAVLGL01.fasta.gz) using Quast (v5.2.0; Mikheenko et al. 2018). This did not include the mitochondrial assembly (OZ075093.1). Busco statistics (Manni et al., 2021) were taken from Höglund et al. (2024) and communication with the authors. Annotation statistics were calculated for pmne_functional_edit1.gff.gz using AGAT (v1.4.1; Dainat, 2024).

BUSCO notation: C: Complete; S: Single-copy; D: Duplicated; F: Fragmented; M: Missing; n: Total BUSCO genes included in the dataset (here: lepidoptera_odb10). See also the official BUSCO manual.

Publication(s)

The data for Parnassius mnemosyne displayed in the genome portal comes from:

Höglund, J., Dias, G., Olsen, R. A., Soares, A., Bunikis, I., Talla, V., & Backström, N. (2024). A Chromosome-Level Genome Assembly and Annotation for the Clouded Apollo Butterfly (Parnassius mnemosyne): A Species of Global Conservation Concern. Genome Biology and Evolution, 16(2), evae031. https://doi.org/10.1093/gbe/evae031

The tools used by to calculate the statistics shown on top of this page are described in:

  • Dainat J. (2024). AGAT: Another Gff Analysis Toolkit to handle annotations in any GTF/GFF format. (Version v1.4.1). Zenodo. https://www.doi.org/10.5281/zenodo.3552717

  • Manni, M., Berkeley, M. R., Seppey, M., Simão, F. A., & Zdobnov, E. M. (2021). BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Molecular Biology and Evolution, 38(10), 4647–4654. https://doi.org/10.1093/molbev/msab199

  • Mikheenko, A., Prjibelski, A., Saveliev, V., Antipov, D., & Gurevich, A. (2018). Versatile genome assembly evaluation with QUAST-LG. Bioinformatics, 34(13), i142–i150. https://doi.org/10.1093/bioinformatics/bty266

Funding

The study in which the genome data was generated (Höglund et al. 2024) was funded by:

  • Swedish Research Council (Grant number 2019-04791)
  • NBIS/SciLifeLab long-term bioinformatics support
  • Swedish Rescue Program for P. mnemosyne through the local administrative board (Länsstyrelsen) of Blekinge

Page last updated: 22/10/2024