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Genome assembly

Species

Littorina saxatilis

Rough periwinkle

Description

  • Assembly Name: US_GU_Lsax_2.0
  • Assembly Type: Haploid
  • Assembly Level: Chromosome
  • Genome Representation: Full
  • Accession: GCA_037325665.1

Assembly Statistics

StatisticValue
Assembly length (Mbp)1254.85
GC %42.35
Contig #10770
Contig N50 (Mbp)0.27
Contig L501289
Contig N90 (Mbp)0.05
Contig L905317
Scaffold #4070
Scaffold N50 (Mbp)67.52
Scaffold L508
Scaffold N90 (Mbp)44.90
Scaffold L9017
Scaffolds >= 10 kb2924
BUSCO % (mollusca_odb10), genomeC:79.2 [S:77.7, D:1.5], F:4.9, M:15.9, n:5295

Annotation Statistics

StatisticValue
Gene #25144
Transcript #29761
Avg exons per transcript7.2
Avg gene length (bp)12995
Avg transcript length (bp)14810
Avg exon length (bp)218
Avg intron length (bp)2145
BUSCO % (mollusca_odb10), predicted proteinsC:82.2 [S:62.5, D:19.7], F:1.2, M:16.6, n:5295

Notes: Assembly statistics were calculated for the primary genome assembly GCA_037325665.1 (GCA_037325665.1_US_GU_Lsax_2.0_genomic.fna.gz) using Quast (v5.2.0; Mikheenko et al. 2018). Busco statistics (Manni et al., 2021) were taken from Supplemental Table S1 in De Jode et al. (2024). Annotation statistics were calculated for GCA_037325665.1_US_GU_Lsax_2.0_genomic.gff.gz using AGAT (v1.4.1; Dainat, 2024).

BUSCO notation: C: Complete; S: Single-copy; D: Duplicated; F: Fragmented; M: Missing; n: Total BUSCO genes included in the dataset (here: mollusca_odb10). See also the official BUSCO manual.

Publication(s)

The data for Littorina saxatilis displayed in the genome portal comes from:

De Jode, A., Faria, R., Formenti, G., Sims, Y., Smith, T. P., Tracey, A., Wood, J. M. D., Zagrodzka, Z. B., Johannesson, K., Butlin, R. K., & Leder, E. H. (2024). Chromosome-scale Genome Assembly of the Rough Periwinkle Littorina saxatilis. Genome Biology and Evolution, 16(4), evae076. https://doi.org/10.1093/gbe/evae076

The tools used by to calculate the statistics shown on top of this page are described in:

  • Dainat J. (2024). AGAT: Another Gff Analysis Toolkit to handle annotations in any GTF/GFF format. (Version v1.4.1). Zenodo. https://www.doi.org/10.5281/zenodo.3552717

  • Manni, M., Berkeley, M. R., Seppey, M., Simão, F. A., & Zdobnov, E. M. (2021). BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Molecular Biology and Evolution, 38(10), 4647–4654. https://doi.org/10.1093/molbev/msab199

  • Mikheenko, A., Prjibelski, A., Saveliev, V., Antipov, D., & Gurevich, A. (2018). Versatile genome assembly evaluation with QUAST-LG. Bioinformatics, 34(13), i142–i150. https://doi.org/10.1093/bioinformatics/bty266

Funding

The study in which the genome data was generated (De Jode et al. 2024) acknowledge funding by:

  • The European Research Council (grant agreement number 693030),
  • The Swedish Research Council VR (grant agreement numbers 2018-03695, 2020-05385, and 2021-04191)
  • The European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie (grant agreement number 706376)
  • The Fundação para a Ciência e a Tecnologia (2020.00275.CEECIND and PTDC/BIA-EVL/1614/2021)

Page last updated: 15/10/2024