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Genome assembly

Species

Bufotes viridis

European green toad

Description

  • Assembly Name: Bufotes_viridis_n_2023_12
  • Assembly Type: Haploid
  • Assembly Level: Scaffold
  • Genome Representation: Full
  • Accession: GCA_963931945.1

Assembly Statistics

StatisticValue
Assembly length (Mbp)3879.46
GC %43.68
Contig #3599
Contig N50 (Mbp)6.73
Contig L50169
Contig N90 (Mbp)0.81
Contig L90761
Scaffold #2107
Scaffold N50 (Mbp)478.30
Scaffold L504
Scaffold N90 (Mbp)162.75
Scaffold L909
Scaffolds >= 10 kb2092
BUSCO % (tetrapoda_odb10)C:91.3 [S:09.4, D:0.9], F:2.4, M:6.3, n:5310

Annotation Statistics

StatisticValue
Gene #31608
Transcript #69426
Avg exons per transcript7.9
Avg gene length (bp)34945
Avg transcript length (bp)38456
Avg exon length (bp)310
Avg intron length (bp)5010

Notes: Assembly statistics were calculated for the primary genome assembly GCA_963931945.1 (CAWYQA01.fasta.gz) using Quast (v5.2.0; Mikheenko et al. 2018). Busco statistics (Manni et al., 2021) were taken from Rödin-Mörch et al. (2025). Annotation statistics were calculated for GCA_963931945.1_Bufotes_viridis_n_2023_12_genomic.gff.gz using AGAT (v1.4.1; Dainat, 2024).

BUSCO notation: C: Complete; S: Single-copy; D: Duplicated; F: Fragmented; M: Missing; n: Total BUSCO genes included in the dataset (here: eudicots_odb10). See also the official BUSCO manual.

Publication(s)

The data for Bufotes viridis displayed in the genome portal comes from:

Rödin-Mörch, P., Bunikis, I., Choi, E., Ciofi, C., Diedericks, G., Diroma, M. A., Einarsdóttir, E., Försäter, K., Heintz, J., Jonsäll, L., Lantz, H., Laurila, A., Leitão, H. G., Mosbech, M.-B., Natali, C., Olsen, R.-A., Vinnere Pettersson, O., Soler, L., Svardal, H., … Höglund, J. (2025). A chromosome-level genome assembly of the European green toad (Bufotes viridis). G3 Genes|Genomes|Genetics, 15(3), jkaf002. https://doi.org/10.1093/g3journal/jkaf002

The tools used by to calculate the statistics shown on top of this page are described in:

  • Dainat J. (2024). AGAT: Another Gff Analysis Toolkit to handle annotations in any GTF/GFF format. (Version v1.4.1). Zenodo. https://www.doi.org/10.5281/zenodo.3552717

  • Manni, M., Berkeley, M. R., Seppey, M., Simão, F. A., & Zdobnov, E. M. (2021). BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Molecular Biology and Evolution, 38(10), 4647–4654. https://doi.org/10.1093/molbev/msab199

  • Mikheenko, A., Prjibelski, A., Saveliev, V., Antipov, D., & Gurevich, A. (2018). Versatile genome assembly evaluation with QUAST-LG. Bioinformatics, 34(13), i142–i150. https://doi.org/10.1093/bioinformatics/bty266

Funding

The study in which the genome data was generated (Rödin-Mörch et al. 2025) acknowledge funding by:

  • Being part of a pilot project run by the European Reference Genome Atlas (ERGA) consortium and included in the Biodiversity Genomics Europe project (Grant no. 101059492) funded by Horizon Europe under the Biodiversity, Circular Economy and Environment call (REA.B.3);
  • The Swiss State Secretariat for Education, Research and Innovation (SERI) under contract numbers 22.00173 and 24.00054
  • The UK Research and Innovation (UKRI) under the Department for Business, Energy and Industrial Strategy’s Horizon Europe Guarantee Scheme.
  • The Swedish partnership in the pilot project was made available by a grant to SciLifeLab from Uppsala University.
  • The use of Agilent, Dovetail, and Illumina kits were kindly sponsored by the manufacturers.

Page last updated: 10/06/2025